The Microarray Core Facility, supported in part by the COBRE Center for Targeted Therapeutics, is a state-of-the-art facility that conducts gene expression profiling to monitor the levels of expression of all genes in the genome.
Using our technology, a single experiment allows researchers to determine specific alterations in gene expression in various disease states, or progression, following exposure to drugs or chemicals. 30,000 genes are expressed at different levels in a single genome, and can be monitored in a single experiment through our technology.
The facility is well equipped to provide custom microarrays for academic and industrial clients. So far we have successfully spotted cDNAs, 50 to 70 base pair oligonucleotides and 18 to 22 base pair short primers. In addition to nucleic acids, the Biochip Arrayer can spot antibodies, proteins, peptides and bacterial cells as well. We produce arrays of the highest quality but at a low density (up to about 2,000 spots per slide).
We assist all clients in designing the appropriate microarray for your needs. We take the guesswork out of array alignment and maintain a high level of spatial precision throughout the printing run. Specification with regard to array design and spot placement is tightly monitored. This attention to detail results in saved scanner time and fewer auto format failures.
We provide extensive on site training and support for all MAUI 4-bay Hybridization System and Agilent’s Hybridization Oven users.
Support is provided for spotted microarray scanning with ScanAray Express SP3 and quantitative analysis with ScanAray Express SP3 and ImaGene software.
We assist customers with gene expression data analysis using limma/limmaGUI and GeneSifter software. Additionally, we perform cluster analysis using Cluster and Treeview software both from Eisen laboratory.
Quantity and quality of RNA and DNA are determined with the help of Agilent 2100 Bioanalyzer.
- Packard BioChip Arrayer
- Packard Multiprobe II Liquid Handler
- MAUI 4-Bay Hybridization System
- Agilent High-Resolution Microarray Scanner
- Agilent Hybridization Oven
- Agilent 2100 Bioanalyzer
- Microarray Links
The Microarray Core Facility receives partial support from SC INBRE, funded by the Institutional Development Award (IDeA) program of the National Center for Research Resources. The Facility is also a member of the Network of IDeA-funded Core Laboratories, (NICL).